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May 4, The techniques and On this Six key elements' analysis of FAC effective management in nuclear power plant. Corporate commitment is the economy base of FAC management and ensure of management system, Analysis is the method of FAC 's optimization and consummation, Operating experience is the reference and complementarity of FAC , Inspection is the base of accumulating FAC data, Training and engineering judgment is the technical complementarity and deepening, Long-term strategy is successful key of FAC management.

Six key elements supplement each other, and make up of a full system of FAC effective management. For present FAC management in our national nuclear power plant, six key elements are the core and bring out the best in each other to found the FAC effective management system and prevent great FAC occurrence. An unresolved need in microbial ecology is methodology to enable quantitative analysis of in situ microbial substrate carbon use at the population level.

Analysis of the taxonomic distribution of the salicylic acid biodegradation trait suggested that PSE population success was not due to conservation of this trait but due to competitiveness for the added carbon. Flow-accelerated corrosion FAC is an environment assisted degradation of structural materials, which usually occurs in pipelines of power plants. There have been many studies to investigate the fundamental mechanism and corresponding countermeasures against FAC , and recently the carbon steels have been replaced by ASTM A P22, which contains approximately 2.

However, while corrosion behavior of Fe-based alloys is relatively well known, their behavior under high-temperature flowing water is not well investigated. In other words, effects of Cr and its corrosion and oxidation behavior is not clearly revealed. Furthermore, it is known that Mo enhances the pitting corrosion resistance of alloys however its mechanism is not clearly investigated. Recently, replacement of Mo in alloy contents has been widely studied because of the cost of Mo. Carbon steels undergo severe environmental-assisted degradation behavior so called FAC , and as its countermeasure the carbon steel has been replaced by P22 which contains Cr and Mo.

It is generally known that Cr and Mo enhances passivity of Fe-based alloys however their corrosion and oxidation behavior has not been fully investigated especially in high-temperature flowing water environments. In this study, we employed HRTEM and synchrotron XAS techniques in order to investigate detailed microstructure evolution and chemical bonding of the commercialized carbon steel and the Fe-Cr-Mo alloys. From the analysis , it is found that while carbon steels exhibit porous oxide P22 exhibit oxide structures with thin Cr-rich oxide and spinel.

Therefore, carbon steel undergoes severe FAC compared to P22 however effects of Cr and Mo and their behavior in high-temperature flowing water will be investigated. Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis.

The two methods can be combined to form a pipeline for cell-line editing that facilitates Directory of Open Access Journals Sweden. Full Text Available We show that high quality microarray gene expression profiles can be obtained following FACS sorting of cells using combinations of transcription factors. Overall, tf FACS represents an advancement in FACS technology which broadly crosses multiple disciplines, most notably in regenerative medicine to redefine cellular populations.

Comparative analysis of circulating endothelial progenitor cells in age-related macular degeneration patients using automated rare cell analysis ARCA and fluorescence activated cell sorting FACS.

FACS has high inter-observer variability and subjectivity when measuring rare cell populations precluding development into a diagnostic test. This pilot study serves as the first step in developing methods for reproducibly predicting ARMD phenotype conversion.

Strict criteria were used to exclude subjects with known angiogenic diseases to minimize confounding results. Blood samples were analyzed in masked fashion in two separate laboratories.

EPC measurements in a small sample. EPCs were independently enumerated using ARCA and FACS within 24 hours of blood sample collection, and ptrend for this proof of concept study, while statistical significance was established at 0. ARCA could be a. Evaluation of LINE-1 mobility in neuroblastoma cells by in vitro retrotransposition reporter assay: FACS analysis can detect only the tip of the iceberg of the inserted L1 elements.

Long Interspersed Nuclear Elements L1 are retroelements generally repressed in most differentiated somatic cells. Their activity has been observed in some undifferentiated and tumour cells and could be involved in tumour onset and progression.

Growing evidences show that the L1 activation can occur in neuronal precursor cells during differentiation process. Neuroblastoma is a tumour originating from neuronal precursor cells, and, although the molecular basis of its progression is still poorly understood, the implication of L1 activation has not yet been investigated. FACS results have shown a low retrotransposition activity.

To detect L1 RP integrated in transcriptionally repressed genomic sites, both a cell treatment with a stimulator of reporter gene promoter, and a quantitative Real-Time PCR analysis were performed. A retrotransposition activity ten and one thousand times that of FACS was found, respectively. These results point out that the real rate of L1 retrotransposition events in tumour cells might be considerably higher than that reported so far by evaluating only the reporter gene expression.

When the labels were removed, Pepsi was the It includes a number of sub-methodologies Conventional methods for detection of infective organisms, such as Salmonella, are complicated and require multiple steps, and the need for rapid detection has increased. Biosensors show great potential for rapid detection of pathogens. In turn, aptamers have great potential for biosensor assay development, given their small size, ease of synthesis and labeling, lack of immunogenicity, a lower cost of production than antibodies, and high target specificity.

In this study, ssDNA aptamers specific to Salmonella Typhimurium were obtained by a whole bacterium-based systematic evolution of ligands by exponential enrichment SELEX procedure and applied to probing S. After 10 rounds of selection with S. Typhimurium as the target and Salmonella Enteritidis, Escherichia coli and Staphylococcus aureus as counter targets, the highly enriched oligonucleic acid pool was sorted using flow cytometry.

In total, 12 aptamer candidates from different families were sequenced and grouped. Fluorescent analysis demonstrated that aptamer C4 had particularly high binding affinity and selectivity; this aptamer was then further characterized. All rights reserved.

Full Text Available Although previous studies of horses have investigated their facial expressions in specific contexts, e. This is essential for an objective description of horse facial expressions across a range of contexts that reflect different emotional states. Facial Action Coding Systems FACS provide a systematic methodology of identifying and coding facial expressions on the basis of underlying facial musculature and muscle movement.

FACS are anatomically based and document all possible facial movements rather than a configuration of movements associated with a particular situation. Consequently, FACS can be applied as a tool for a wide range of research questions. We developed FACS for the domestic horse Equus caballus through anatomical investigation of the underlying musculature and subsequent analysis of naturally occurring behaviour captured on high quality video.

Discrete facial movements were identified and described in terms of the underlying muscle contractions, in correspondence with previous FACS systems. The reliability of others to be able to learn this system Equi FACS and consistently code behavioural sequences was high--and this included people with no previous experience of horses.

A wide range of facial movements were identified, including many that are also seen in primates and other domestic animals dogs and cats. Equi FACS provides a method that can now be used to document the facial movements associated with different social contexts and thus to address questions relevant to understanding social cognition and comparative psychology, as well as informing current veterinary and animal welfare practices.

Lessons learned from fossil FAC assessments. Dooley, R. Barry; Shields, Kevin J. In their work the authors have noted great diversity in the Flow-Accelerated Corrosion FAC Programs used at conventional fossil power plants.

The results and findings of FAC Program assessments conducted at 22 conventional plants are summarized and discussed. By comparing the FAC Program characteristics and relevant unit features with damage and failure experiences, a number of common factors requiring attention from fossil utility organizations have been identified.

The assessment experiences have also provided a picture of trends in specific FAC activities and general awareness within the conventional fossil fleet. One of the most important aspects of these studies is that while a few new locations of FAC have been found, there is some consolidation of the most frequently found locations. Effect of Cr content on the FAC of pipe material at Flow accelerated corrosion FAC of the carbon steel piping in nuclear power plants NPPs has been major issue in nuclear industry.

During the FAC , a protective oxide layer on carbon steel dissolves into flowing water leading to a thinning of the oxide layer and accelerating corrosion of base material. As a result, severe failures may occur in the piping and equipment of NPPs. The facility is equipped with on line monitoring of pH, conductivity, dissolved oxygen DO and temperature. Test solution was the demineralized water, and DO concentration was less than 1 ppb. Surface appearance of A Gr.

The materials with Cr content higher than 0. The pit is thought to early degradation mode of FAC. A functional miniature microfluidics instrument with the ability to remotely distinguish live or dead bacterial cells from abiotic particulates in ice or permafrost of icy bodies of the solar system would be of fundamental value to NASA.

The use of molecular probes to obtain the bio-signature of living or dead cells could answer the most fundamental question of Astrobiology: Does life exist beyond Earth? The live-dead fluorescent stains to be used in the micro FACS instrument function only with biological cell walls. The detection of the cell membranes of living or dead bacteria unlike PAH's and many other Biomarkers would provide convincing evidence of present or past life.

This miniature device rapidly examine large numbers of particulates from a polar ice or permafrost sample and distinguish living from dead bacteria cells and biological cells from mineral grains and abiotic particulates and sort the cells and particulates based on a staining system. Any sample found to exhibit fluorescence consistent with living cells could then be used in conjunction with a chiral labeled release experiment or video microscopy system to seek addition evidence for cellular metabolism or motility.

Results of preliminary testing and calibration of the micro FACS prototype instrument system with pure cultures and enrichment assemblages of microbial extremophiles will be reported.

We performed a statistic study of FAC cases observed by the four Cluster spacecraft in the northern hemisphere. There is almost no correlation or a weak positive correlation of the poleward boundary and IMF cone angle no matter IMF is northward or southward. The equatorward boundary is more responsive to the IMF cone angle. Compared to the equatorward boundary, the center of the FAC projected location changes very little.

Fat stigmatization in television shows and movies: a content analysis. To examine the phenomenon of fat stigmatization messages presented in television shows and movies, a content analysis was used to quantify and categorize fat-specific commentary and humor.

Fat stigmatization vignettes were identified using a targeted sampling procedure, and scenes were excised from movies and television shows. The material was coded by trained raters. Reliability indices were uniformly high for the seven categories percentage agreement ranged from 0. Results indicated that fat stigmatization commentary and fat humor were often verbal, directed toward another person, and often presented directly in the presence of the overweight target. Results also indicated that male characters were three times more likely to engage in fat stigmatization commentary or fat humor than female characters.

To our knowledge, these findings provide the first information regarding the specific gender, age, and types of fat stigmatization that occur frequently in movies and television shows.



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